| Prologue: Camp Bisenzio ITT, 2.2 km | Time | Gap |
| 1 Marianne Vos (Ned) Rabobank liv Giant | 2:53.19 | 0:00 |
| 2 Annamiek Van Vleuten (Ned) Rabobank liv Giant | 2:56.26 | 0:03.07 |
| 3 Shelley Olds (USA) Team TIBCO - To The Top | 2:58.02 | 0:04.83 |
| 4 Kirsten Wild (Ned) Team Argos Shimano | 2:58.89 | 0:05.7 |
| 5 Tiffany Cromwell (Aus) Orica AIS | 2:59.18 | 0:05.99 |
| 6 Valentina Scandolara (Ita) Cipollini Giordana Galassia | 2:59.31 | 0:06.12 |
| 7 Emily Collins (NZl) Wiggle Honda | 3:00.48 | 0:07.29 |
| 8 Pauline Ferrand Prevot (Fra) Rabobank liv Giant | 3:01.13 | 0:07.94 |
| 9 Lauren Hall (USA) Optum p/b Kelly Benefit Strategies | 3:01.17 | 0:07.98 |
| 10 Emma Johansson (Swe) Orica AIS | 3:01.19 | 0:08.00 |
| 11 Alison Powers (USA) US National Team | 3:01.24 | 0:08.05 |
| 12 Thalita De Jong (Ned) Rabobank liv Giant | 3:01.30 | 0:08.11 |
| 13 Anna Van Der Breggen (Ned) Sengers Ladies Cycling Team | 3:01.33 | 0:08.14 |
| 14 Roxanne Knetemann (Ned) Rabobank liv Giant | 3:01.42 | 0:08.23 |
| 15 Lucinda Brand (Ned) Rabobank liv Giant | 3:02.01 | 0:08.82 |
| 16 Inga Cilvinaite (Ltu) Pasta Zara Cogeas | 3:02.57 | 0:09.38 |
| 17 Tatiana Guderzo (Ita) Cipollini Giordana Galassia | 3:02.63 | 0:09.44 |
| 18 Linda Melanie Villumsen (NZl) Wiggle Honda | 3:02.76 | 0:09.57 |
| 19 Noemi Cantele (Ita) BePink | 3:02.80 | 0:09.61 |
| 20 Ester Fenner (Ger) German National Team | 3:02.84 | 0:09.65 |
| 21 Amanda Miller (USA) Team TIBCO - To The Top | 3:03.15 | 0:09.96 |
| 22 Amy Pieters (Ned) Team Argos Shimano | 3:03.40 | 0:10.21 |
| 23 Jade Wilcoxson (USA) Optum p/b Kelly Benefit Strategies | 3:03.70 | 0:10.51 |
| 24 Denise Ramsden (Can) Optum p/b Kelly Benefit Strategies | 3:03.71 | 0:10.52 |
| 25 Charlotte Becker (Ger) Wiggle Honda | 3:03.76 | 0:10.57 |
| 26 Chantal Blaak (Ned) Team TIBCO - To The Top | 3:04.06 | 0:10.87 |
| 27 Evelyn Stevens (USA) Specialized - lululemon | 3:04.13 | 0:10.94 |
| 28 Vera Koedooder (Ned) Sengers Ladies Cycling Team | 3:04.33 | 0:11.14 |
| 29 Emilia Fahlin (Swe) Hitec Products UCK | 3:04.39 | 0:11.2 |
| 30 Jessie Maclean (Aus) Orica AIS | 3:04.48 | 0:11.29 |
| 31 Giorgia Bronzini (Ita) Wiggle Honda | 3:04.49 | 0:11.3 |
| 32 Elisa Longo Borghini (Ita) Hitec Products UCK | 3:04.53 | 0:11.34 |
| 33 Audrey Cordon (Fra) French National Team | 3:05.04 | 0:11.85 |
| 34 Elke Gebhardt (Ger) Team Argos Shimano | 3:05.17 | 0:11.98 |
| 35 Valentina Carretta (Ita) Cipollini Giordana Galassia | 3:05.19 | 0:12.00 |
| 36 Trixi Worrack (Ger) Specialized - lululemon | 3:05.22 | 0:12.03 |
| 37 Tatiana Antoshina (Rus) Cipollini Giordana Galassia | 3:05.32 | 0:12.13 |
| 38 Simona Frapporti (Ita) BePink | 3:05.49 | 0:12.30 |
| 39 Leah Kirchmann (Can) Optum p/b Kelly Benefit Strategies | 3:05.72 | 0:12.53 |
| 40 Kristin Mcgrath (USA) US National Team | 3:05.74 | 0:12.55 |
| 41 Rossella Ratto (Ita) Hitec Products UCK | 3:05.79 | 0:12.60 |
| 42 Jessie Daams (Bel) Boels Dolmans Cycling Team | 3:05.81 | 0:12.62 |
| 43 Aude Biannic (Fra) Michela Fanini Rox | 3:06.18 | 0:12.99 |
| 44 Adrie Visser (Ned) Boels Dolmans Cycling Team | 3:06.34 | 0:13.15 |
| 45 Marta Tagliaferro (Ita) Cipollini Giordana Galassia | 3:06.57 | 0:13.38 |
| 46 Janel Holcomb (USA) Optum p/b Kelly Benefit Strategies | 3:06.77 | 0:13.58 |
| 47 Ingrid Drexel (Mex) Pasta Zara Cogeas | 3:06.77 | 0:13.58 |
| 48 Elena Cecchini (Ita) Faren Kuota | 3:06.93 | 0:13.74 |
| 49 Chloe Hosking (Aus) Hitec Products UCK | 3:07.00 | 0:13.81 |
| 50 Lauren Kitchen (Aus) Wiggle Honda | 3:07.02 | 0:13.83 |
| 51 Claudia Hausler (Ger) Team TIBCO - To The Top | 3:07.37 | 0:14.18 |
| 52 Anna Bianca Schnitzmeier (Ger) Wiggle Honda | 3:07.68 | 0:14.49 |
| 53 Maaike Polspoel (Bel) Sengers Ladies Cycling Team | 3:07.78 | 0:14.59 |
| 54 Maura Kinsella (USA) US National Team | 3:07.93 | 0:14.74 |
| 55 Sofie De Vuyst (Bel) Sengers Ladies Cycling Team | 3:08.02 | 0:14.83 |
| 56 Alexis Ryan (USA) US National Team | 3:08.03 | 0:14.84 |
| 57 Emma Trott (GBr) Boels Dolmans Cycling Team | 3:08.16 | 0:14.97 |
| 58 Sungeun Gu (Kor) Orica AIS | 3:08.23 | 0:15.04 |
| 59 Maria Giulia Confalonieri (Ita) Faren Kuota | 3:08.90 | 0:15.71 |
| 60 Irene Bitto (Ita) Top Girl Fassa Bortolo | 3:08.96 | 0:15.77 |
| 61 Francesca Pattaro (Ita) Italian National Team | 3:09.02 | 0:15.83 |
| 62 Katarzyna Sosna (Ltu) Vaiano Fondriest | 3:09.29 | 0:16.10 |
| 63 Elise Delzenne (Fra) French National Team | 3:09.49 | 0:16.30 |
| 64 Tetyana Riabchenko (Ukr) Chirio Forno D'asolo | 3:09.62 | 0:16.43 |
| 65 Alessandra D'ettorre (Ita) Vaiano Fondriest | 3:09.77 | 0:16.58 |
| 66 Edita Janeliunaite (Ltu) Pasta Zara Cogeas | 3:09.77 | 0:16.58 |
| 67 Polona Batagelj (Slo) Slovenian National Team | 3:10.02 | 0:16.83 |
| 68 Alena Sitsko (Blr) Chirio Forno D'asolo | 3:10.15 | 0:16.96 |
| 69 Silvia Valsecchi (Ita) BePink | 3:10.45 | 0:17.26 |
| 70 Barbara Guarischi (Ita) Vaiano Fondriest | 3:10.46 | 0:17.27 |
| 71 Lara Vieceli (Ita) Michela Fanini Rox | 3:10.77 | 0:17.58 |
| 72 Arianna Fidanza (Ita) Italian National Team | 3:10.79 | 0:17.6 |
| 73 Sara Frece (Slo) Slovenian National Team | 3:10.85 | 0:17.66 |
| 74 Christel Ferrier Bruneau (Fra) Faren Kuota | 3:10.94 | 0:17.75 |
| 75 Nina Kessler (Ned) Boels Dolmans Cycling Team | 3:11.05 | 0:17.86 |
| 76 Lauren Stephens (USA) Team TIBCO - To The Top | 3:11.14 | 0:17.95 |
| 77 Edwige Pitel (Fra) Michela Fanini Rox | 3:11.31 | 0:18.12 |
| 78 Lucy Garner (GBr) Team Argos Shimano | 3:11.70 | 0:18.51 |
| 79 Elena Berlato (Ita) Top Girl Fassa Bortolo | 3:11.90 | 0:18.71 |
| 80 Yevgenia Wysotska (Ukr) Michela Fanini Rox | 3:12.21 | 0:19.02 |
| 81 Amy Cure (Aus) Orica AIS | 3:12.27 | 0:19.08 |
| 82 Valentina Bastianelli (Ita) Vaiano Fondriest | 3:12.29 | 0:19.10 |
| 83 Janneke Busser (Ned) Team Argos Shimano | 3:12.29 | 0:19.10 |
| 84 Liisi Rist (Est) Michela Fanini Rox | 3:12.39 | 0:19.20 |
| 85 Romy Kasper (Ger) Boels Dolmans Cycling Team | 3:12.61 | 0:19.42 |
| 86 Clemilda Fernandes (Bra) Chirio Forno D'asolo | 3:12.71 | 0:19.52 |
| 87 Serika Guluma (Col) Vaiano Fondriest | 3:12.77 | 0:19.58 |
| 88 Agne Silinyte (Ltu) Pasta Zara Cogeas | 3:12.81 | 0:19.62 |
| 89 Amelie Rivat (Fra) French National Team | 3:12.99 | 0:19.80 |
| 90 Elizabeth Wilborne (USA) US National Team | 3:13.04 | 0:19.85 |
| 91 Katie Collclough (GBr) Specialized - lululemon | 3:13.21 | 0:20.02 |
| 92 Rachel Neylan (Aus) Hitec Products UCK | 3:13.31 | 0:20.12 |
| 93 Kelly Markus (Ned) Team Argos Shimano | 3:13.54 | 0:20.35 |
| 94 Susanna Zorzi (Ita) Faren Kuota | 3:14.09 | 0:20.90 |
| 95 Julia Soek (Ned) Sengers Ladies Cycling Team | 3:14.51 | 0:21.32 |
| 96 Melanie Bravard (Fra) French National Team | 3:14.78 | 0:21.59 |
| 97 Ilaria Bonomi (Ita) Italian National Team | 3:14.94 | 0:21.75 |
| 98 Martina Ruzickova (Cze) Pasta Zara Cogeas | 3:15.29 | 0:22.10 |
| 99 Miriam Bjornsrud (Nor) Hitec Products UCK | 3:15.48 | 0:22.29 |
| 100 Malgorzata Jasinska (Pol) Cipollini Giordana Galassia | 3:15.51 | 0:22.32 |
| 101 Fabiana Luperini (Ita) Faren Kuota | 3:15.98 | 0:22.79 |
| 102 Spela Kern (Slo) Slovenian National Team | 3:16.44 | 0:23.25 |
| 103 Alice Maria Arzuffi (Ita) BePink | 3:16.55 | 0:23.36 |
| 104 Annalisa Cucinotta (Ita) Servetto Footon | 3:16.65 | 0:23.46 |
| 105 Elizabeth Armitstead (GBr) Boels Dolmans Cycling Team | 3:17.24 | 0:24.05 |
| 106 Michele Maltese (Ita) Italian National Team | 3:17.39 | 0:24.20 |
| 107 Ursa Pintar (Slo) Slovenian National Team | 3:17.63 | 0:24.44 |
| 108 Alexia Muffat (Fra) French National Team | 3:17.98 | 0:24.79 |
| 109 Olm Shekel (Ukr) Chirio Forno D'asolo | 3:18.17 | 0:24.98 |
| 110 Jutatip Maneephan (Tha) Michela Fanini Rox | 3:18.43 | 0:25.24 |
| 111 Andrea Dvorak (USA) US National Team | 3:18.44 | 0:25.25 |
| 112 Ajda Opeka (Slo) Slovenian National Team | 3:18.48 | 0:25.29 |
| 113 Giada Borgato (Ita) Pasta Zara Cogeas | 3:18.57 | 0:25.38 |
| 114 Stephanie Pohl (Ger) German National Team | 3:18.59 | 0:25.40 |
| 115 Dalia Muccioli (Ita) BePink | 3:18.93 | 0:25.74 |
| 116 Marina Lari (Ita) Servetto Footon | 3:19.51 | 0:26.32 |
| 117 Nicole Dal Santo (Ita) Italian National Team | 3:19.53 | 0:26.34 |
| 118 Chiara Pierobon (Ita) Top Girl Fassa Bortolo | 3:19.82 | 0:26.63 |
| 119 Lisa Kullmer (Ger) German National Team | 3:20.19 | 0:27.00 |
| 120 Lisa Fischer (Ger) German National Team | 3:20.21 | 0:27.02 |
| 121 Alessia Martini (Ita) Vaiano Fondriest | 3:20.55 | 0:27.36 |
| 122 Julia Martisova (Rus) Chirio Forno D'asolo | 3:20.58 | 0:27.39 |
| 123 Asja Paladin (Ita) Top Girl Fassa Bortolo | 3:20.71 | 0:27.52 |
| 124 Marion Sicot (Fra) French National Team | 3:21.32 | 0:28.13 |
| 125 Alenka Novak (Slo) Slovenian National Team | 3:21.50 | 0:28.31 |
| 126 Francesca Cauz (Ita) Top Girl Fassa Bortolo | 3:21.51 | 0:28.32 |
| 127 Veronica Kormos (Bel) Sengers Ladies Cycling Team | 3:21.52 | 0:28.33 |
| 128 Petra Zrimsek (Slo) BePink | 3:21.71 | 0:28.52 |
| 129 Miriam Romei (Ita) Italian National Team | 3:21.91 | 0:28.72 |
| 130 Uenia Fernandes Da Souza (Bra) Chirio Forno D'asolo | 3:22.13 | 0:28.94 |
| 131 Simona Bortolotti (Ita) Servetto Footon | 3:25.13 | 0:31.94 |
| 132 Sarah Lena Hofmann (Ger) German National Team | 3:26.76 | 0:33.57 |
| 133 Corinna Defile' (Ita) Servetto Footon | 3:27.48 | 0:34.29 |
| 134 Marie Therese Ludwig (Ger) German National Team | 3:27.96 | 0:34.77 |
| 135 Joelle Numainville (Can) Optum p/b Kelly Benefit Strategies | 3:30.18 | 0:36.99 |
| 136 Tayler Wiles (USA) Specialized - lululemon | 3:30.47 | 0:37.28 |
| 137 Emma Marcelli (Ita) Servetto Footon | 3:32.94 | 0:39.75 |